Characteristics of Medium Proteins:
One,
For Humans:
Proteomics research ultimately aims to serve human health. It plays a key role in advancing molecular medicine. For example, it helps identify target molecules for drug development. Many drugs are proteins themselves, and the targets of these drugs are often proteins as well. Additionally, drugs can interfere with protein-protein interactions, making proteomics an essential field in drug discovery and development.
Two,
Post-Translational Modification:
Many proteins undergo post-translational modifications after being expressed from mRNA. These include phosphorylation, glycosylation, and zymogen activation. Such modifications are crucial for regulating protein function, so studying them is vital for understanding how proteins work within cells.
Three,
Protein Identification:
Proteins can be identified using techniques such as one-dimensional or two-dimensional electrophoresis combined with Western blotting. Other methods include protein chips, antibody chips, and immunoprecipitation. These approaches help researchers detect and analyze specific proteins in complex biological samples.
Four,
Function Determination of Medium Proteins:
To determine protein function, scientists analyze enzyme activity, identify substrates, and perform ligand-receptor binding assays for cytokines. Gene knockout and antisense techniques also allow researchers to study the functional impact of gene expression. Moreover, determining where a protein localizes within a cell can provide critical insights into its role. Clontech's fluorescent protein expression system is a powerful tool for visualizing protein localization in living cells.
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